Ibidi

A Pump System for the Cultivation of, e.g. Endothelial, Cells Under Flow for Simulation of Blood Vessels

Product outline:

• A pump system for the cultivation of, e.g. endothelial, cells under flow for simulation of blood vessels.
• Ideal simulation of various physiological conditions – continuous unidirectional, oscillating, and pulsatile flow.
• Compatible with the ibidi Heating Systems, all incubators, incubated microscopes, and Channel µ-Slides.
• Minimal mechanical stress, minimal amount of medium and supplement needed.

Product outline:

• Stage Top Incubator for easy live cell imaging on every inverted microscope: Universal Fit with CO2 and O2 control
• Physiologic conditions directly on the microscope
• Precise control of temperature, humidity, CO2, and O2
• Compatible with all inverted microscopes that have a multiwell plate holder

Product outline:

• A µ-Plate with 96 wells to investigate angiogenesis in high throughput tube formation assays
• A high throughput screening plate with full ANSI/SLAS (SBS) and robotics compatibility
• Brilliant cell visualization without gel meniscus formation
• Cost-effective high throughput experiments, requiring only 10 µl of gel per well
• Similar to µ-Slide Angiogenesis but with 96 wells in a multiwell plate format

Applications

• High throughput tube formation assays
• 3D cell culture
• Immunofluorescence staining
• Sprouting assays
• Live cell imaging of attached cells

Product outline:

• A µ-Plate with 96 wells to investigate angiogenesis in high throughput tube formation assays
• A high throughput screening plate with full ANSI/SLAS (SBS) and robotics compatibility
• Brilliant cell visualization without gel meniscus formation
• Cost-effective high throughput experiments, requiring only 10 µl of gel per well
• Similar to µ-Slide Angiogenesis but with 96 wells in a multiwell plate format

Applications:

• Cultivation and high-resolution microscopy of cells
• Immunofluorescence staining and fluorescence microscopy of living and fixed cells
• Live cell imaging over extended time periods
• Transfection assays
• Differential interference contrast (DIC) microscopy when used with a DIC lid

Product outline:

• A µ-Slide with 18 wells for use in matrix tests and quick immunofluorescence staining
• Low volume screening slide for medium throughput
• Cost-effective experiments using small numbers of cells and low volumes of reagents
• Compatible with multi-channel pipettes

Applications

• Quick immunofluorescence staining of adherent cells
• Optimization of both surface functionalization’s and coatings
• Fast toxicological screening of small microscopy samples
• Spotting samples such as RNA assays
• Cultivation of small organisms

Product outline:

• A chambered coverslip with 8 wells for cell culture, immunofluorescence, and high-end microscopy
• All-in-one 8 well chamber slide for cost-effective experiments—small number of cells and low volume of reagents needed
• In this microscopy slide, the cells are imaged on a No. 1.5 polymer coverslip bottom with the highest optical quality
• A cell culture chamber suitable for most microscopy techniques (e.g., DIC, widefield fluorescence, confocal microscopy, two-photon microscopy, FRAP, FRET, FLIM, or LSFM)

Applications

• Immunofluorescence staining
• Live cell imaging over extended time periods
• Transfection assays
• Differential interference contrast (DIC) when using a DIC lid

Surface Modification: #1.5 (170µm + 20/-10µm) D 263 M Schott glass sterilized.

Product outline:

• A chambered coverslip with 8 individual wells, high walls, and a #1.5H glass coverslip bottom, suitable for use in TIRF and single molecule applications
• A cell culture chamber for observation of the sample through a glass coverslip bottom using high resolution microscopy
• Brilliant cell imaging thanks to the low thickness variability of the coverslips’ glass
• Cost-effective experiments using small numbers of cells and low volumes of reagents
• Extra high individual walls to keep cross contamination between wells as low as possible

Applications

• Cultivation and high-resolution microscopy of cells
• TIRF and single molecule applications of living and fixed cells
• Super-resolution microscopy
• Immunofluorescence staining and fluorescence microscopy of living and fixed cells
• Live cell imaging over extended time periods
• Transfection assays
• Differential interference contrast (DIC) microscopy when used with a DIC lid

Product outline:

• A µ-Slide used to investigate angiogenesis in tube formation assays. Also ideal for 3D cell culture and immunofluorescence staining.
• Complete solution for tube formation experiments, requiring only a few steps from sample preparation to image analysis
• Brilliant visualization without meniscus formation, and with all cells in one focal plane
• Can be used with a broad range of gels (e.g., Matrigel®, collagen, and agarose)
• Cost-effective experiments, requiring only 10 µl of gel per well

Applications

• Tube formation assays
• 3D cell culture
• Immunofluorescence staining
• Sprouting assays
• Live cell imaging of attached cells

Product outline:

• Investigate chemotaxis of fast or slow migrating adherent cells and non-adherent cells in 2D or 3D gel matrices
• Real-time chemotaxis measurement in real-time in a 2D or 3D environment
• Stable chemotactic gradients for long-term experiments
• Reproducible results with reliable and user-independent data

Applications

• 2D and 3D chemotaxis assays with fast or slow migrating cells
• Live cell imaging using inverted microscopy
• Chemotaxis of neutrophils, lymphocytes, and monocytes
• 3D chemotaxis of leukocytes or cancer cells in an ECM-like matrix
• Invasion assays of tumour cells in Matrigel™
• Chemotaxis measurements of adherent and non-adherent cells

Product outline:

• Channel slides with different heights, volumes, and coatings especially suited for flow applications
• Large area of uniform shear stress
• Easy connection using Luer adapters
• Homogeneous cell distribution with defined optical pathway

Applications

• Adherent cells under flow conditions
• Cell culture (static or stop-flow)
• Simulation of blood vessels with endothelial cells
• High-resolution microscopy of living and fixed cells

A µ-Slide With One Channel and Three Wells for Culturing Cells on a 3D Gel Matrix With Defined Flow

Product outline:

• Create a perfusable cell monolayer on a gel matrix for various flow and shear stress applications
• Establish and image endothelial barriers under in vivo-like conditions—no artificial filters or membranes
• Easy handling: samples are placed into the open wells; perfusion is applied after closing the wells

Applications

• Culture of adherent cells on a 3D gel matrix under flow conditions
• Transendothelial migration studies under flow conditions
• Establishment and microscopic read-out of endothelial barrier assays (without any artificial membrane)
• Simulation of blood vessels with endothelial cell culture on a soft substrate
• Long-term or short-term cell culture perfusion experiments with defined shear stress and optimal nutrient supply into 3D structures
• Apical-basal cell polarity assays
• Cell barrier model assays with apical-basal gradients
• Cell boundary assays without filters or porous membranes
• Rolling and adhesion assays using leukocytes
• Inflammation studies
• Transport studies

Product Outline:

• A µ-Slide with a porous glass membrane for transmigration and transport studies under both static and flow conditions
• Brilliant optical quality through the thin, porous glass membrane
• Full access to the apical and basal sides of adherent cells in numerous applications
• Different pore sizes available for various cell types
• Suitable for the establishment of lung models with air-liquid interface (ALI)

Applications

• Trans-endothelial migration under flow conditions
• Co-cultivation of cell layers and transport in 2D or in a 3D gel matrix
• Apical-basal cell polarity assays
• Skin and lung models with liquid-air interface
• Cell barrier model assays with apical-basal gradients
• Cell migration assays based on filters and porous membranes