Particle Metrix – ZetaView
Biological nanoparticles such as extracellular vesicles (EVs), exosomes, viruses, or virus-like particles are a rapidly growing area of research in the life sciences and nanomedical field. The rapid in vitro measurement of multiple physical parameters such as size, concentration, surface charge, and phenotype characteristics is the special feature of the Particle Metrix ZetaView®.
Nanoparticle Tracking Analysis (NTA) captures the Brownian motion of each particle in the video. Based on the different diffusion movements of large and small particles in the surrounding liquid, the hydrodynamic diameter of the particles is determined. Furthermore, the charge state of the particle surface (zeta potential) can be measured via the movement of the particles in an applied electric field.
Pattern parameters, such as intensity fluctuations, surface geometry and shape of the particles as well as particle concentration are documented at each recording and can be used to distinguish sub-populations.
All these analyzes are carried out quickly and statistically reliably as required in the scattered light or fluorescence mode.
Available Models
Key Features
- Very short measuring times
- “Ready to use” calibration standard
- 11 measuring positions, optionally combined with dosing steps
- Outlier free result
- Sample recovery
- Low Bleach Technology
- Fully automatic focusing of the optics
- Quartz cell channel and easy cleaning
- SOP methodology
- Applicable in the GMP range
- IQ / OQ / PQ
Key Features
- Very short measuring times
- “Ready to use” calibration standard
- 11 measuring positions, optionally combined with dosing steps
- Outlier free result
- Sample recovery
- Low Bleach Technology
- Fully automatic focusing of the optics
- Quartz cell channel and easy cleaning
- SOP methodology
- Applicable in the GMP range
- IQ / OQ / PQ
- 2 fluorescence channels per sample
- Laser and fluorescence channels switched per mouse click
Key Features
- Very short measuring times
- “Ready to use” calibration standard
- 11 measuring positions, optionally combined with dosing steps
- Outlier free result
- Sample recovery
- Low Bleach Technology
- Fully automatic focusing of the optics
- Quartz cell channel and easy cleaning
- SOP methodology
- Applicable in the GMP range
- IQ / OQ / PQ
- 4 fluorescence channels per sample
- Laser and fluorescence channels switched per mouse click
- Upgradable from PMX-120 BASIC to PMX-220 TWIN or PMX-420 QUATT
Operating ZetaView
Resources
Download Application Notes
- Application notes – Identification of DNA / RNA containing Extracellular Vesicles with DAPI and Particle Metrix ZetaView® Nanoparticle Tracking
- Application notes – Identification of platelet-derived Exosomes in plasma samples with Particle Metrix ZetaView® Nanoparticle Tracking
- Application notes – Purity check of Extracellular Vesicle preparations by Particle Metrix ZetaView® Nanoparticle Tracking Analysis
Download Tech Notes
- Tech notes – Reliability of concentration measurements with the Particle Metrix ZetaView® Nanoparticle Tracking Analyzer
- Tech notes – Limitation of sample volume: How to recover a sample after NTA measurement
- Tech notes – Zeta potential and size measurement of nanoparticles by laser scattering video microscopy
Brand
The company headquarter of Particle Metrix is located in Meerbusch near Dusseldorf, whereas production and development resides in Inning near Munich. Customers have at their disposal a competent team of chemists, physicists, biologists and engineers. It focuses on Nanoparticle Tracking Analysis (NTA) as a method for rapid and comprehensive characterization of extracellular vesicles (EVs), viruses and similar small biological particles. Our ZetaView® NTA devices provide complementary information on flow cytometry (FC) but in the lower nanometer range. The overlap of FC and NTA is typically between 100 nm and 300 nm.