Stilla Technologies – Digital PCR – Naica 6 colors system
The Naica® System
Get absolute quantification of multiple genetic targets in a single run
Digital PCR (dPCR) is a powerful tool used for the precise and highly sensitive quantification of nucleic acids.
The naica® system is an easy-to-use dPCR platform that harnesses cutting-edge microfluidic technology to integrate the dPCR workflow onto a single consumable chip. The technology, known as Crystal Digital PCR™, partitions samples into a large array of thousands of individual droplet crystals – each its own reaction compartment – before amplifying nucleic acid molecules in each droplet crystal. These reactions are tagged with fluorophores to be read using up to six different fluorescence light channels.
This makes for a fast and simple workflow that provides even more sensitivity while reducing time to results and saving precious sample.
Key Features
Six Fluorescent Channels
The accurate and precise quantification of multiple targets in a single reaction saves you both time and precious sample. The naica® system gives researchers the ability to read up to six fluorescent signatures in one well, providing better sensitivity and increased low-level detection of multiple reactions in parallel.
User-friendly Crystal Miner Software
Crystal Miner Software provides intuitive visuals for image analysis, allowing you to not only explore your data but to also see your data by inspecting droplet crystals for quality control. Crystal Miner Software measures the concentrations of targeted nucleic acids, providing automatic identification of positive and negative droplet crystals for all fluorescence channels.
Greater Precision, Accuracy, and Sensitivity
Digital PCR has proven its adaptability in numerous molecular assays and is now recognized as a reliable method not only for rare alleles and rare mutations detection but also for genotyping, absolute quantification, and copy number variation.
The naica system has been successfully implemented in the wide variety of applications, including:
- Cancer and liquid biopsy
- Cell and gene therapy
- Infection disease detection
- GMO and environmental testing
Simple to Use
With a combination of powerful imaging, the flexibility to use up to six detection channels, and a proprietary software for analysis, the naica® system brings simplicity to digital PCR.
The naica® system is comprised of:
- naica® system dPCR mixes and reagents kits
- Consumable chips (Sapphire or Opal)
- Geode (automated droplet generator and thermocycler)
- Prism3 or Prism6 (multi-color fluorescence imager)
- Crystal Miner data analysis (software)
Featured Products
SapphireChip
Up to 12 samples / run Up to 30,000 droplets / sample For high sensitivity applications
Opal Chip
Up to 48 samples / run Up to 20,000 droplets / sample For flexible throughput
Geode
Thermocycler for partitioning and amplification
EGFR 6-color Crystal Digital PCR™ Kit
The EGFR 6-color kit allows for the detection and quantification of up to 32 somatic EGFR mutations in exons 18, 19, 20, and 21 associated with non-small cell cancer (NSCLC). The kit can be used on cell-free DNA (cfDNA) extracted from plasma samples.
Prism6
High multiplexing capacity 3- or 6-color system configuration Wide range of fluorophore compatibility
Crystal Miner Software
Intuitive software for visual inspection and analysis
6-color Naica® System Workflow
The 6-color naica® system is a complete digital PCR solution with flexible sample throughput, and a unique 6-color target detection capability which enables another exclusive dimension for multiplexed dPCR assays while maintaining an easy-to-use workflow.
Brand
At Stilla Technologies, we continuously innovate and evolve technologically toward better human health outcomes. Our global team with multidisciplinary expertise shares a passion to develop new products for advanced genetic testing and analysis. Our expertise spans from microfluidics and chemistry to molecular biology and computer science. Our product, the naica® System, has been adopted by researchers & clinicians around the world to develop a wide range of genetic tests and molecular biology assays – including, but not limited to, liquid biopsy tests for cancer diagnostics, infectious diseases, prenatal testing, and GMO detection.
