Product outline:

• A µ-Plate with 96 wells to investigate angiogenesis in high throughput tube formation assays
• A high throughput screening plate with full ANSI/SLAS (SBS) and robotics compatibility
• Brilliant cell visualization without gel meniscus formation
• Cost-effective high throughput experiments, requiring only 10 µl of gel per well
• Similar to µ-Slide Angiogenesis but with 96 wells in a multiwell plate format

Applications

• High throughput tube formation assays
• 3D cell culture
• Immunofluorescence staining
• Sprouting assays
• Live cell imaging of attached cells

Product outline:

• A µ-Plate with 96 wells to investigate angiogenesis in high throughput tube formation assays
• A high throughput screening plate with full ANSI/SLAS (SBS) and robotics compatibility
• Brilliant cell visualization without gel meniscus formation
• Cost-effective high throughput experiments, requiring only 10 µl of gel per well
• Similar to µ-Slide Angiogenesis but with 96 wells in a multiwell plate format

Applications:

• Cultivation and high-resolution microscopy of cells
• Immunofluorescence staining and fluorescence microscopy of living and fixed cells
• Live cell imaging over extended time periods
• Transfection assays
• Differential interference contrast (DIC) microscopy when used with a DIC lid

Product outline:

• A µ-Slide with 18 wells for use in matrix tests and quick immunofluorescence staining
• Low volume screening slide for medium throughput
• Cost-effective experiments using small numbers of cells and low volumes of reagents
• Compatible with multi-channel pipettes

Applications

• Quick immunofluorescence staining of adherent cells
• Optimization of both surface functionalization’s and coatings
• Fast toxicological screening of small microscopy samples
• Spotting samples such as RNA assays
• Cultivation of small organisms

Product outline:

• A chambered coverslip with 8 wells for cell culture, immunofluorescence, and high-end microscopy
• All-in-one 8 well chamber slide for cost-effective experiments—small number of cells and low volume of reagents needed
• In this microscopy slide, the cells are imaged on a No. 1.5 polymer coverslip bottom with the highest optical quality
• A cell culture chamber suitable for most microscopy techniques (e.g., DIC, widefield fluorescence, confocal microscopy, two-photon microscopy, FRAP, FRET, FLIM, or LSFM)

Applications

• Immunofluorescence staining
• Live cell imaging over extended time periods
• Transfection assays
• Differential interference contrast (DIC) when using a DIC lid

Surface Modification: #1.5 (170µm + 20/-10µm) D 263 M Schott glass sterilized.

Product outline:

• A chambered coverslip with 8 individual wells, high walls, and a #1.5H glass coverslip bottom, suitable for use in TIRF and single molecule applications
• A cell culture chamber for observation of the sample through a glass coverslip bottom using high resolution microscopy
• Brilliant cell imaging thanks to the low thickness variability of the coverslips’ glass
• Cost-effective experiments using small numbers of cells and low volumes of reagents
• Extra high individual walls to keep cross contamination between wells as low as possible

Applications

• Cultivation and high-resolution microscopy of cells
• TIRF and single molecule applications of living and fixed cells
• Super-resolution microscopy
• Immunofluorescence staining and fluorescence microscopy of living and fixed cells
• Live cell imaging over extended time periods
• Transfection assays
• Differential interference contrast (DIC) microscopy when used with a DIC lid

Product outline:

• A µ-Slide used to investigate angiogenesis in tube formation assays. Also ideal for 3D cell culture and immunofluorescence staining.
• Complete solution for tube formation experiments, requiring only a few steps from sample preparation to image analysis
• Brilliant visualization without meniscus formation, and with all cells in one focal plane
• Can be used with a broad range of gels (e.g., Matrigel®, collagen, and agarose)
• Cost-effective experiments, requiring only 10 µl of gel per well

Applications

• Tube formation assays
• 3D cell culture
• Immunofluorescence staining
• Sprouting assays
• Live cell imaging of attached cells

Product outline:

• Investigate chemotaxis of fast or slow migrating adherent cells and non-adherent cells in 2D or 3D gel matrices
• Real-time chemotaxis measurement in real-time in a 2D or 3D environment
• Stable chemotactic gradients for long-term experiments
• Reproducible results with reliable and user-independent data

Applications

• 2D and 3D chemotaxis assays with fast or slow migrating cells
• Live cell imaging using inverted microscopy
• Chemotaxis of neutrophils, lymphocytes, and monocytes
• 3D chemotaxis of leukocytes or cancer cells in an ECM-like matrix
• Invasion assays of tumour cells in Matrigel™
• Chemotaxis measurements of adherent and non-adherent cells

Product outline:

• Channel slides with different heights, volumes, and coatings especially suited for flow applications
• Large area of uniform shear stress
• Easy connection using Luer adapters
• Homogeneous cell distribution with defined optical pathway

Applications

• Adherent cells under flow conditions
• Cell culture (static or stop-flow)
• Simulation of blood vessels with endothelial cells
• High-resolution microscopy of living and fixed cells

A µ-Slide With One Channel and Three Wells for Culturing Cells on a 3D Gel Matrix With Defined Flow

Product outline:

• Create a perfusable cell monolayer on a gel matrix for various flow and shear stress applications
• Establish and image endothelial barriers under in vivo-like conditions—no artificial filters or membranes
• Easy handling: samples are placed into the open wells; perfusion is applied after closing the wells

Applications

• Culture of adherent cells on a 3D gel matrix under flow conditions
• Transendothelial migration studies under flow conditions
• Establishment and microscopic read-out of endothelial barrier assays (without any artificial membrane)
• Simulation of blood vessels with endothelial cell culture on a soft substrate
• Long-term or short-term cell culture perfusion experiments with defined shear stress and optimal nutrient supply into 3D structures
• Apical-basal cell polarity assays
• Cell barrier model assays with apical-basal gradients
• Cell boundary assays without filters or porous membranes
• Rolling and adhesion assays using leukocytes
• Inflammation studies
• Transport studies

Product Outline:

• A µ-Slide with a porous glass membrane for transmigration and transport studies under both static and flow conditions
• Brilliant optical quality through the thin, porous glass membrane
• Full access to the apical and basal sides of adherent cells in numerous applications
• Different pore sizes available for various cell types
• Suitable for the establishment of lung models with air-liquid interface (ALI)

Applications

• Trans-endothelial migration under flow conditions
• Co-cultivation of cell layers and transport in 2D or in a 3D gel matrix
• Apical-basal cell polarity assays
• Skin and lung models with liquid-air interface
• Cell barrier model assays with apical-basal gradients
• Cell migration assays based on filters and porous membranes

A Perfusable Channel µ-Slide With 3 x 7 Wells for Long-Term Spheroid Cultivation and High-End Microscopy

Product outline:

• Ideal for the long-term cultivation and perfusion of 3D spheroids or organoids on the Bioinert surface
• Flexible preparation for perfusion: spheroids can be transferred or created directly in the wells
• Imaging chamber with excellent optical quality for high-resolution microscopy

Applications

• Cultivation and high-resolution microscopy of three-dimensional cell aggregates (e.g., spheroids and organoids)
• For use with the ibidi Pump System or any other pump device with Luer connectors
• Perfusion of spheroids for fresh medium supply during long-term cultivation and access to spheroid growth kinetics
• Spheroid generation directly in the wells
• Spheroid retrieval for downstream processing (e.g., immunofluorescence, histology, and biochemical assays)
• Perfusion of cell monolayers (adherent cells), suspension cells, or co-cultures
• Organ-on-a-chip setups with up- and downstream metabolization
• Live cell imaging and microscopy of 3D cell aggregates
• Immunofluorescence staining and high-resolution fluorescence microscopy of living and fixed cells and cell aggregates

Nanoscale Imaging Has Never Been Easier

Collect nanoscale topography data on your benchtop in three clicks with the nGauge AFM.

ICSPI has integrated all of the components of a traditional AFM onto a single 1 mm x 1 mm chip to create the world’s first single-chip AFM. That means that 250 AFMs can fit on the face of a penny.

The nGauge AFM uses micro-electro-mechanical systems (MEMS) to control the X, Y and Z position of the tip. This means that no alignment of lasers is required.

The nGauge is the smallest AFM in the world. Because the entire unit weighs less than 500 grams, the nGauge rejects building vibrations, so no vibration isolation table is needed, which makes the nGauge a true benchtop AFM.

 

What can I use AFM for?

AFM provides topographical data of a surface. That means that you can look at the shape and size of individual features, such as the pits on a DVD, or look at the particle density, such as the number of nanoparticles in an area.

The nGauge AFM can be used to investigate surfaces where the features are up to 10 µm tall. It’s tricky to pinpoint a lower limit, but the RMS noise in the vertical (z) direction of the nGauge is 1 nanometre (nm). So, depending on your requirements, features as small as 5–10 nm can be imaged with the nGauge with acceptable accuracy.

To put what a nanometer is into perspective, a quarter (25-cent coin) is about 2 cm wide. An E. coli bacterium cell is 2 µm wide (10,000× smaller than a quarter). And the diameter of a DNA helix is 2 nm (1,000× smaller than a bacterium).

PCR products are manufactured, from prime virgin, polypropylenes. This result in tubes, strips and, tips that exhibit a perfect balance between transparency, softness, robustness, antistatic characteristics and gas tightness.